LOCUS       pLV-[hsa-miR-1323]        9127 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7048..7908
                     /label=Amp(R)
     promoter        8109..8649
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5653..5887
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6462..6917)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5959..6089
                     /label=SV40 pA
     misc_feature    6090..6303
                     /label=SV40 ori
     misc_feature    4974..5570
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3592
                     /label=intron
     CDS             3639..4964
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3382
                     /label=hsa-mir-1323 precursor
BASE COUNT     2161 a      2370 c      2473 g      2123 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct gagtgctgtg 
     3121 tgaacgtcct ccatctgcag tgtgaggaca gcgaacccta gtgtgagtta accacgtagg 
     3181 aagagtttga agtcagacat gacattcaga ctgaggtcct caaaactgag gggcattttc 
     3241 tgtggtttga aaggaaagtg cacccagttt tggggatgtc aattgtgaat cctcatcata 
     3301 acccatcccc agtcccccat cgcattgcac cacatggatg cacccagtgg actggccttt 
     3361 gcttttctat tgtttctttt tcgctagctc gagcttttgg agtacgtcgt ctttaggttg 
     3421 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 
     3481 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 
     3541 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 
     3601 aaaactaccc ctctagagtc gagctaccgg tcgccaccat ggtgagcaag ggcgaggagg 
     3661 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 
     3721 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 
     3781 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 
     3841 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 
     3901 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 
     3961 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga 
     4021 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 
     4081 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 
     4141 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 
     4201 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 
     4261 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 
     4321 ccggcggcat ggacgagctg tacaaggacc caccggtcgc caccatgacc gagtacaagc 
     4381 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 
     4441 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 
     4501 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 
     4561 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 
     4621 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 
     4681 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 
     4741 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 
     4801 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 
     4861 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gagtgcccga 
     4921 aggaccgcgc gacctggtgc atgacccgca agcccggtgc ctgagcggcc gcgtcgacaa 
     4981 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 
     5041 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 
     5101 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 
     5161 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 
     5221 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 
     5281 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 
     5341 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 
     5401 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 
     5461 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 
     5521 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc 
     5581 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 
     5641 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct 
     5701 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 
     5761 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 
     5821 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 
     5881 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 
     5941 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     6001 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     6061 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 
     6121 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     6181 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 
     6241 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 
     6301 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 
     6361 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 
     6421 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 
     6481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     6541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     6601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     6661 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     6721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     6781 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 
     6841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     6901 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     6961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     7021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     7081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     7141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     7201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     7261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     7321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     7381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     7441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     7501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     7561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     7621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     7681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     7741 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     7801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     7861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     7921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     7981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     8041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     8101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     8161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     8221 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     8281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     8341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     8401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     8461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     8521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     8581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     8641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     8701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 
     8761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 
     8821 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 
     8881 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 
     8941 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 
     9001 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 
     9061 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 
     9121 gagctgc 
//
